Heart Biomarker Evaluation in Apnea Treatment

4.3.6.2 AM Draw

1. **SERUM** After centrifugation, 1.0 mL of the serum is pipetted into cryovials # 1- 4. Tube # 1 is a clear-capped 4.0 mL cryovial and tubes # 2- 4 are red-capped 1.5 mL cryovials.
2. **CITRATE** After centrifugation, 0.5 mL of plasma is pipetted into cryovials # 5 - 8 (see Aliquoting Scheme Chart). These will be blue-capped cryovials. The packed cells remaining in the tube can be added to tube # 17. Tube # 17 is a red-capped 10.0 mL tube.
3. **EDTA** After centrifugation, 1.0 mL plasma will be pipetted into cryovials # 9 - 12 as specified in the Aliquoting Scheme Chart. These will be purple-capped cryovials. The packed cells remaining in the tube are added to tube # 17.
4. **SCAT-1** After centrifugation, plasma will be pipetted into cryovials # 13 - 16 (see Aliquoting Scheme Chart). These will be yellow-capped cryovials. The packed cells remaining in the tube are added to tube # 17.
5. **CPT** See appendix for processing. Plasma will be pipetted into green-capped cryovials # 18 – 19. Cells will be pipetted into clear-capped cryovials # 20 - 21.
6. **Packed Cells** The packed cell tube # 17 is a red-capped cryovial that will be frozen and shipped to the LCBR for storage. Although the tube holds 10 mL, only 9 mL of material can be added to the tube due to expansion during freezing. The cells from the EDTA and SCAT-1 tubes should be added to tube # 17 first and, if there is sufficient space, cells from the citrate tube can also be added for a total volume of 9 mL.

Once the samples are aliquoted, the EDTA, Citrate and SCAT-1 blood collection tubes can be discarded in the biohazard waste (puncture-proof containers).