Childhood Adenotonsillectomy Trial

4.1.2 Equipment and Supplies

The following supplies are recommended by the LCBR:

• Aliquots - 1.5 mL skirted cryovials, 4.0mL tall tubes,
• 9.0 mL tube with colored caps
• Sarstedt 4.0 mL cryovial (for Lipid Profile): Catalog # 62.611 Fisher Scientific:

USA Scientific 1.5 cryovials and caps for blood samples:

• purple caps Catalog # 1470-0505
• red caps Catalog # 1470-0504
• 1.5mL cryovials Catalog # 1415-4700

Blood collection requires the following supplies:

• Lab coats and gloves
• Basin (just in case)
• Washcloths/Towels
• Plastic cart with wheels for phlebotomy supplies (or plastic tray with compartments)
• Butterfly needles (21 G) with luer adapter (Butterfly with safety needle 21G ¾ x 12", B-D Vacutainer 367281)
• Vacutainer barrels
• Tourniquets
• Alcohol prep pads
• Gauze (2x2)
• Surgical tape - paper tape (easier on participants)
• Band-Aids
• Blood tube racks
• Scissors
• Pens
• Labels and Phlebotomy Form
• Biohazardous waste container
• Needle/sharps container
• Ice bucket and crushed ice - filled 10 min before draw
• Stopwatches or timers
• Blood collection tubes (keep extras on hand):
  • 1 –10 mL EDTA tubes
  • 1 –10 mL Serum tubes

The blood processing area should have the following additional supplies and equipment:

• Lab mats and wipes
• Blood tube rocker
• 10% bleach solution or approved biohazard disinfectant
• Refrigerated centrifuge (4°C) capable of spinning at a minimum of 2000 x g
• -80°C freezer (or colder) or
• -20°C freezer and dry ice
• Refrigerator