Cleveland Family Study

14.1 Calibration

14.1.1 Start Mode Procedure

1. Connect the RS-232 cable to the laptop
2. Turn on nitric oxide analyzer power using switch on back. Warm-up time is 15 minutes
3. Connect regulator to oxygen outlet and set to 10 PSIG. This is already set in Room 685 and is usually connected to the wall.
4. From main menu, Press Clear to display status screen
5. Verify the following:
   • Cooler Temp -12 ± 0.2 PSIG (Adjust black knob on back)
   • Supply pressure 6.0 ± 0.2 PSIG
   • Cell Pressure >300 torr
6. Press Clear to return to main menu
7. With the Analyzer option highlighted, press Enter
8. From the analyzer menu, select Start. The NOA must be in start mode to perform a calibration

14.1.2 Calibration Gas Procedure

Two or three gas calibrations should be performed to make sure that the calibration is not drifting. Replicated gas calibrations should agree to the third decimal place

1. Connect sample tubes to one leg of the tee.
2. Open valve on calibration gas cylinder. No minimum, just open. This is the harder tube used in calibration.
3. From the Calibration Menu, select Calib Gas to display the instruction screen
4. Allow the NOA to draw calibration gas through the machine for approximately 2-5 minutes.
5. You may start calibration after 2 minutes
6. Press Enter to display the gas concentration screen.
7. The default concentration of calibration gas is 45 ppm. Press Enter to accept the default.
8. Select PPM&PPB to calibrate both ranges.
9. Press Enter to accept first calibration.
10. Repeat two-three times. Must be within .002.
11. TURN OFF THE GAS TANK.
12. Disconnect the tubing from the tank.
13. Leave the machine running for testing.